Identification of the substancepreparation and of the companyundertaking product name t4 dna ligase product no m0202 recommended use of the chemical and restrictions on use. Unlike the full length ligase, t4 rnl2 truncated is unable to adenylate the. Comparative analysis of the endjoining activity of several dna ligases. All enzymes and buffers were obtained from new england biolabs neb, ipswich, ma unless otherwise noted. This product is covered by one or more patents, trademarks andor s owned or controlled by new england biolabs, inc neb. For those product lots not listed below, please contact neb at email protected or fill out the technical support form for appropriate document. T4 rna ligase 2 t4 rna ligase 2 truncated t4 rna ligase.
The following protocol is for rapid ligation of cohesive ends. Optimization of golden gate assembly through application of. Thermo scientific t4 dna ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5phosphate and 3hydroxyl termini in duplex dna or. The increased concentration of reaction components is helpful in lower volume reactions where reagent volumes are limited. Promega corporation 2800 woods hollow roadmadison, wi 537115399 u. Use nebcloner to find the right products and protocols for each in your traditional cloning workflow, including double digestion buffers. Unlike t4 rna ligase 1, t4 rna ligase 2 is much more active joining nicks on double stranded rna than on joining the ends of single stranded rna. T4 dna ligase competitor study nuclease contamination t4 dna ligase from multiple suppliers was tested in reactions containing a fluorescent labeled single stranded, double stranded blunt, 3overhang or 5 overhang containing oligonucleotides. New england biolabs uk ltd t4 rna ligase reaction buffer. Download a pdf containing pricing for our full product list. Dna ligases and ligase master mixes new england biolabs gmbh.
New england biolabs uk ltd bluntta ligase master mix. This master mix is specifically formulated to improve ligation and transformation of both bluntend and singlebase overhang substrates. T4 rna ligase 1, high concentration m0437m, is a 3x higher concentration than our standard t4 rna ligase 1 m0204sl. Cohesive ends, blunt ends, and nick sealing can all be efficiently catalyzed by t3 dna ligase.
View a rna ligase selection chart outlining neb s rna ligases and recommendations for optimal performance. T3 dna ligase is an atpdependent ds dna ligase from bacteriophage t3. For details on neb s quality controls for dna ligases, visit our ligase quality page. Toll free in the usa 8003569526 telephone 6082744330 internet usage information i. T4 rna ligase 2 t4 rna ligase 2 truncated t4 rna ligase 2, truncated k227q. Choose a dna, rna, genome editing, qpcr calculator from neb, a leader in production and supply of reagents for the life science industry.
The quick ligation kit enables ligation of cohesive end or blunt end dna fragments in 5 minutes at room temperature. This module provides reagents for the ligation step of the standardoriginal library prep workflow. For high temperatures, we recommend taq dna ligase. T4 dna ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5 phosphate and. New england biolabs supplies a 10x reaction buffer with all of its enzymes at 1x concentration this reaction buffer assures optimal activity of the enzyme. Thermo scientific t4 dna ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5phosphate and 3hydroxyl termini in duplex dna or rna. Yields of final ligation product for all reaction conditions using high concentration t4 dna ligase. Product listing application overview neb continues to develop and produce the most extensive commercially available selection of highquality. Ligation protocol with t4 dna ligase m0202 protocols. A thermotolerant variant of t4 dna ligase, hi t4 dna ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5. Products neb catalogue rna t4 rna ligase reaction buffer. Set up the following reaction in a microcentrifuge tube on ice. Neb now offers a second kit for golden gate assembly featuring bsmbiv2 and a destination plasmid for use in any.
Rapid ligation of cohesive ends 5min for plasmid cloning of dna fragments. This product is packaged with a vial of 100 mm atp n0451a. Yields of final ligation product for all reaction conditions using high concentration t4 dna ligase, the quick ligation kit and bluntta master mix. This higher concentration is required for rapid ligation. While neb develops and validates its products for various applications, the use of this product may require the buyer to obtain additional. Dna ligases and ligase master mixes stick together. For rapid ligation of blunt ends, use t4 dna ligase, cat no. Dna ligases are key enzymes in molecular and synthetic biology that catalyze the. Enzyme activity in cutsmart buffer required supplements.
Neb s t4 dna ligase contains less contaminating activity to learn more about how neb s bluntta ligase master mix outperforms other ligases on difficult to ligate substrates, download our application notes at yields of final ligation product for. T4 dna ligase catalyzes the formation of phosphodiester bonds in the presence of atp between doublestranded dnas with 3 hydroxyl and 5 phosphate termini. The ligation of mismatched overhangs leads to erroneous assembly. T4 dna ligase is the industry standard for performance and quality at a 1x concentration this reaction buffer assures optimal activity of the enzyme. Yields of final ligation product for all reaction conditions using high concentration t4 dna ligase, the quick ligation kit and bluntta master.
Comprehensive profiling of four base overhang ligation fidelity by. T4 rnl2 truncated is expressed from a plasmid in e. The following link is to a pdf safety data sheet sds that applies to this product to help you use it safely. To save your cart and view previous orders, sign in to your neb. Bluntta ligase master mix is a readytouse solution of t4 dna ligase, proprietary ligation enhancer, and optimized reaction buffer. New england biolabs uk ltd t4 rna ligase 2, truncated.
Identification of the substancepreparation and of the companyundertaking product name t4 dna ligase. The enzyme does not require atp for ligation but does need the preadenylated substrate. This module can be combined with other nebnext modules to cover the entire library prep workflow, or with. The unique t4 dna ligase buffer optimizes ligation. Safety data sheet document type aghs osha ghs revision date 21jul2016 version 3 1. Order t4 dna ligase close order t4 dna ligase reaction buffer close order t7 dna ligase. T4 rna ligase 1 ssrna ligase, high concentration neb. Bluntta ligase master mix outperforms other ligases on difficult to ligate substrates, download our application notes at.
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